Haynes Lab:Notebook/Jan/2015/03/28: Difference between revisions

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(Autocreate 2015/03/28 Entry for Haynes_Lab:Notebook/Jan)
 
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==Entry title==
==03/28/15==
* Insert content here...
* Minipreps - BD006/MV2 colonies (from 3/26/15 ligations), 5 mL each (4 total)
 
 
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'''Minipreps - BD006/MV2 colonies'''
*'''[[User:Karmella Haynes|---Karmella]] 14:00, 28 March 2015 (EDT)''':
 
* Sigma GenElute kit.  
** Elute & back-elute w/ 70 μL elution sln.
 
 
* Restriction Digest check
** Cut with ApaLI (E/S insertion got rid of BB RFC23 cut sites)





Revision as of 11:00, 28 March 2015

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03/28/15

  • Minipreps - BD006/MV2 colonies (from 3/26/15 ligations), 5 mL each (4 total)



Minipreps - BD006/MV2 colonies

  • Sigma GenElute kit.
    • Elute & back-elute w/ 70 μL elution sln.


  • Restriction Digest check
    • Cut with ApaLI (E/S insertion got rid of BB RFC23 cut sites)