Haynes Lab:Notebook/Jan/2015/12/04: Difference between revisions
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* '''Designed primers to amplify three overlapping regions across the transgenes: Chromosensor1, Chromosensor 2''' | * '''Designed primers to amplify three overlapping regions across the transgenes: Chromosensor1, Chromosensor 2''' | ||
# chrsn1 f1/ chrsn1 r1; Validation region 1; sensor 1 (and 2) | |||
# chrsn1 f2/ chrsn1 r2; Validation region 1; sensor 1 (and 2) | |||
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* '''Round 2: Genomic DNA prepped from candidate clonal lines''' | * '''Round 2: Genomic DNA prepped from candidate clonal lines''' | ||
** To be done after chosing a single primer pair per validation region | |||
# BD###, Chromosensor 1 | # BD###, Chromosensor 1 | ||
# BD###, Chromosensor 1 | # BD###, Chromosensor 1 |
Revision as of 16:16, 3 December 2015
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Entry title
PCR - validation of Chromatin sensor insert
ROUND 1 PCR: Neg, pos controls
Note: *Prep one Mix per template
Thermal cycler: Labnet - GOTAQ
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