Haynes Lab:Notebook/Jan/2016/06/21: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
(4 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
Line 14: Line 14:
| bgcolor=#cfcfcf | Vol
| bgcolor=#cfcfcf | Vol
| bgcolor=#cfcfcf | Mix* (x #)
| bgcolor=#cfcfcf | Mix* (x #)
| rowspan=7 | Expected:<br>1. CMV = 588
| rowspan=7 | Expected:<br>1. 4-x, P1 = 1145 <br>2. 4-x, P3 = 1046 <br>3. 4-x, P6 = 1139 <br>4. 6-x, P1 = 1146 <br>5. 6-x, P3 = 1046 <br>6. 6-x, P6 = 1146
| rowspan=7 | [[Image:somegel.jpg|150px|Hover name]]<br>5 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan=7 | [[Image:20161-06-21_Verification_gel_1.jpg|300px|Hover name]]<br>[[Image:20161-06-21_Verification_gel_2.jpg|300px|Hover name]]<br>5 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
| genomic OR plasmid DNA || 1.0 / 0.5  || 4.0 / 2.0
| genomic OR plasmid DNA || 1.0 / 0.5  || 4.0 / 2.0
Line 41: Line 41:


* Conclusions
* Conclusions
 
*Top image, top row, left to right: 4-4, 4-5, 6-1, 6-2, P1, 3, and 6 for each.
*Top image, bottom row, 6-3, 6-4, 6-5, 6-6, P1, 3, and 6 for each.
*Bottom image, top row, 6-7, P1, 3, and 6.
*Bottom image samples on bottom row are something else.




Latest revision as of 01:49, 27 September 2017

Project name Main project page
Previous entry      Next entry

Redo of PCR/Gel Electrophoresis for 4-4, 4-5, 6-1, 6-2, 6-3, 6-4, 6-5, 6-6, 6-7 and Redo of Flow Cytometry Analysis on Synthetic Repressor Experiment

  • PCR: Candidate cell lines (3 primers + 1 error = 4)
Reagent Vol Mix* (x #) Expected:
1. 4-x, P1 = 1145
2. 4-x, P3 = 1046
3. 4-x, P6 = 1139
4. 6-x, P1 = 1146
5. 6-x, P3 = 1046
6. 6-x, P6 = 1146 		Hover name
Hover name
5 μL/lane; 1% agarose; Ladder
genomic OR plasmid DNA 1.0 / 0.5 4.0 / 2.0
10 μM forward primer 1.0 ---
10 μM reverse primer 1.0 ---
2x GoTaq green 10.0 40.0
dH2O 7.0 / 7.5 28.0 / 30
  20.0 μL 72.0 μL

Note: *Prep one Mix per template

  • For each template, aliquot 18.0 master mix into 3 tubes

Thermal cycler: Labnet - GOTAQ

  • 95°C 3 min.
  • 30x[95°C, 30 sec; 57°C 30 sec; 72°C 30 sec]
  • 72°C 3 min.
  • 4°C, ∞


  • Conclusions
  • Top image, top row, left to right: 4-4, 4-5, 6-1, 6-2, P1, 3, and 6 for each.
  • Top image, bottom row, 6-3, 6-4, 6-5, 6-6, P1, 3, and 6 for each.
  • Bottom image, top row, 6-7, P1, 3, and 6.
  • Bottom image samples on bottom row are something else.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Jan/2016/06/21&oldid=1018356"