Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Notes)
(Notes)
Line 19: Line 19:
==Notes==
==Notes==
-
* Transformation of DH5
+
* Transformation of DH5 was successful. Colonies were growing in agar+amp gel.
-
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/25 6/25/13 for protocol]
+
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/25 6/25/13]
-
* Example: This project is currently on hold until further notice.
+
*Yield from miniprep was low at 5.542ng/μL. Expected yield was from 20-80ng/μL. Yield reading may have been due to contamination.
 +
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/28#Spectrophotometer_analysis 6/28/13]

Revision as of 22:23, 2 July 2013

Search this Project

Customize your entry pages

Project Description

This notebook consists of the procedures learnt during lab training. DH5α will be transformed with KAH013 plasmid, and then miniprep will be performed, and a spectrophotometer will be used to analyze the DNA yield, and if the process was successful.

Notes

  • Transformation of DH5 was successful. Colonies were growing in agar+amp gel.

6/25/13

  • Yield from miniprep was low at 5.542ng/μL. Expected yield was from 20-80ng/μL. Yield reading may have been due to contamination.

6/28/13


Recent changes



Personal tools