Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi

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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/28#Spectrophotometer_analysis 6/28/13]
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/28#Spectrophotometer_analysis 6/28/13]
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*Plasmid was validated using restriction digest and electrophoresis.
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*Plasmid was successfully validated using restriction digest and electrophoresis.
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/02 7/02/13]
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/02 7/02/13]

Revision as of 03:03, 8 July 2013

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Project Description

This notebook consists of the procedures learnt during lab training. DH5α will be transformed with KAH013 plasmid, and then miniprep will be performed, and a spectrophotometer will be used to analyze the DNA yield, and if the process was successful. In order to validate the plasmid, restriction digest and electrophoresis will be employed.

Notes

  • Transformation of DH5α was successful. Colonies were growing in agar+amp gel.

6/25/13

  • Yield from miniprep was low at 5.542ng/μL. Expected yield was from 20-80ng/μL. Yield reading may have been due to contamination.

6/28/13

  • Plasmid was successfully validated using restriction digest and electrophoresis.

7/02/13

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