Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi

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(Notes)
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*Plasmid was successfully validated using restriction digest and electrophoresis.
*Plasmid was successfully validated using restriction digest and electrophoresis.
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/02 7/02/13]
[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/02 7/02/13]
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*DH5α was transformed, but was incorrectly grown in LB-amp plates instead of LB+amp plates.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/24 7/24/13]
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*DH5α was transformed, and was correctly grown in LB+amp plates.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/27 7/26/13]
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*Colony was selected from the plate, and transferred to liquid media.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/27 7/27/13]
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Revision as of 18:12, 4 August 2013

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Project Description

This notebook consists of the procedures learnt during lab training. DH5α will be transformed with KAH013 plasmid, and then miniprep will be performed, and a spectrophotometer will be used to analyze the DNA yield, and if the process was successful. In order to validate the plasmid, restriction digest and electrophoresis will be employed.

Notes

  • Transformation of DH5α was successful. Colonies were growing in agar+amp gel.

6/25/13

  • Yield from miniprep was low at 5.542ng/μL. Expected yield was from 20-80ng/μL. Yield reading may have been due to contamination.

6/28/13

  • Plasmid was successfully validated using restriction digest and electrophoresis.

7/02/13

  • DH5α was transformed, but was incorrectly grown in LB-amp plates instead of LB+amp plates.

7/24/13

  • DH5α was transformed, and was correctly grown in LB+amp plates.

7/26/13

  • Colony was selected from the plate, and transferred to liquid media.

7/27/13


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