Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/06/25: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/06/25 Entry for Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==DH5α Transformation with KAH013 Plasmid 6/25/2013==
* Insert content here...
#In ice bath, 1μl KAH013 plasmid and 50μl competent E.Coli were combined.
 
#Waited 10 minutes for bacteria to accept plasmid.
#Transformed bacteria were then added to Agarose gel petri dish, rolling beads were used to evenly spread bacteria.
#Petri dish was labeled with initials date and plasmid name("PK 6/25/2013 KAH13") and was placed in incubator at 37 degrees for two days(6/25-6/26).
#Resultant colonies were picked by Rene and grown in liquid LB media overnight(6/27/2013).


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 22:53, 26 September 2017

Project name Main project page
Next entry

DH5α Transformation with KAH013 Plasmid 6/25/2013

  1. In ice bath, 1μl KAH013 plasmid and 50μl competent E.Coli were combined.
  2. Waited 10 minutes for bacteria to accept plasmid.
  3. Transformed bacteria were then added to Agarose gel petri dish, rolling beads were used to evenly spread bacteria.
  4. Petri dish was labeled with initials date and plasmid name("PK 6/25/2013 KAH13") and was placed in incubator at 37 degrees for two days(6/25-6/26).
  5. Resultant colonies were picked by Rene and grown in liquid LB media overnight(6/27/2013).


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/25&oldid=1012850"