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Project name
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DH5α Transformation with KAH013 Plasmid 6/25/2013
- In ice bath, 1μl KAH013 plasmid and 50μl competent E.Coli were combined.
- Waited 10 minutes for bacteria to accept plasmid.
- Transformed bacteria were then added to Agarose gel petri dish, rolling beads were used to evenly spread bacteria.
- Petri dish was labeled with initials date and plasmid name("PK 6/25/2013 KAH13") and was placed in incubator at 37 degrees for two days(6/25-6/26).
- Resultant colonies were picked by Rene and grown in liquid LB media overnight(6/27/2013).
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