Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/02: Difference between revisions
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(Autocreate 2013/07/02 Entry for Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi) |
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== | ==Restriction Digest== | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> | |||
|- | |||
| ChIP enriched DNA || 30.0 μL | |||
|- | |||
| dH<sub>2</sub>O || 10.0 | |||
|- | |||
| T4 DNA ligase buffer || 5.0 | |||
|- | |||
| dNTP mix || 2.0 | |||
|- | |||
| T4 DNA Polymerase || 1.0 | |||
|- | |||
| Klenow DNA polymerase || 1.0 | |||
|- | |||
| T4 PNK || 1.0 | |||
|- | |||
| || 20 μL | |||
|} | |||
Revision as of 23:10, 7 July 2013
 Project name
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Restriction Digest
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