Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/02: Difference between revisions
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#Fill both sides of the electrophoresis apparatus with 1xTAE to the indicated line. | #Fill both sides of the electrophoresis apparatus with 1xTAE to the indicated line. | ||
#Then remove comb. | #Then remove comb. | ||
<u></u><br> | <u>Perform Electrophoresis</u><br> | ||
#Using a micropipette, add 10μL of Ladder into the left-most well. Make sure no bubbles form in the pipette tip. | |||
#When adding DNA to wells, angle the micropipette, and try make sure that the DNA does not leave the well. | |||
#Add 10μL of KAH013 DNA into a different well. | |||
*Order follwed of DNA placed in wells was: Ladder, David's plasmid (KAH06), Prad's plasmid (KAH013), and Heather's Plasmid(KAH014) | |||
#Make sure that the black and red pins are correctly connected to the apparatus, black towards the wells, and red towards the other side. | |||
#Set apparatus to 100V, and press run. | |||
#Wait 1 hour. | |||
#Gently pull gel out of the apparatus taking care not to tear it, and dry it and place it in paper towels. | |||
#Place gel under UV light, and close the lid on the UV light. Record needed observations, and remove the gel. | |||
#Clean off UV light. | |||
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Revision as of 23:55, 7 July 2013
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Restriction Digest
ElectrophoresisPerparation of Gel
Preparation of Gel Plates
Perform Electrophoresis
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