Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/02

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(Autocreate 2013/07/02 Entry for Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi)
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==Entry title==
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==Restriction Digest==
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{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
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|-valign="top"
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| <u>Reagent</u> || <u>Volume</u>
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|-
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| ChIP enriched DNA || 30.0 μL
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|-
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| dH<sub>2</sub>O || 10.0
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|-
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| T4 DNA ligase buffer || 5.0
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|-
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| dNTP mix || 2.0
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|-
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| T4 DNA Polymerase || 1.0
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|-
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| Klenow DNA polymerase || 1.0
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|-
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| T4 PNK || 1.0
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|-
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| &nbsp; || 20 μL
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|}
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Revision as of 02:10, 8 July 2013

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Restriction Digest

Reagent Volume
ChIP enriched DNA 30.0 μL
dH2O 10.0
T4 DNA ligase buffer 5.0
dNTP mix 2.0
T4 DNA Polymerase 1.0
Klenow DNA polymerase 1.0
T4 PNK 1.0
  20 μL



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