Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/02

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(Entry title)
(Restriction Digest)
Line 12: Line 12:
| <u>Reagent</u> || <u>Volume</u>
| <u>Reagent</u> || <u>Volume</u>
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|-
-
| ChIP enriched DNA || 30.0 μL  
+
| DNA- KAH013 Plasmid || 10.0 μL  
|-
|-
-
| dH<sub>2</sub>O || 10.0  
+
| dH<sub>2</sub>O || 6.0  
|-
|-
-
| T4 DNA ligase buffer || 5.0  
+
| 10x Fast Digest Buffer || 2.0  
|-
|-
-
| dNTP mix || 2.0  
+
| X Enzyme || 1.0  
|-
|-
-
| T4 DNA Polymerase || 1.0
+
| P Enzyme || 1.0  
-
|-
+
-
| Klenow DNA polymerase || 1.0
+
-
|-
+
-
| T4 PNK || 1.0  
+
|-
|-
| &nbsp; || 20 μL
| &nbsp; || 20 μL

Revision as of 02:12, 8 July 2013

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Restriction Digest

Reagent Volume
DNA- KAH013 Plasmid 10.0 μL
dH2O 6.0
10x Fast Digest Buffer 2.0
X Enzyme 1.0
P Enzyme 1.0
  20 μL



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