Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/02: Difference between revisions
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==Electrophoresis== | ==Electrophoresis== | ||
<u>Perparation of Gel</u><br> | |||
#Add 0.6g of agarose to the flask specifically labeled for preparation of agarose gel. (Add agarose first so that it does not stick to the sides of the flask) | |||
#Add 60mL of 1xTAE buffer, and mix thoroughly with agarose. | |||
#Microwave flask for 30s. | |||
#Remove the flask from the microwave using hot hands, point it away from you and swirl. Make sure to swirl away the buildup on the sides of the flask. | |||
#Microwave for 30s again, and repeat the swirling. | |||
#Wait for agarose gel liquid to cool. | |||
<u>Preparation of Gel Plates</u><br> | |||
#Make sure you are wearing gloves and a lab coat, you will be handling ethidium bromide! | |||
#Take ethidium bromide out of fridge, and with a paper towel open the ethidium bromide. | |||
#Add 6μL ethidium bromide to the agar liquid in flask, and swirl carefully. | |||
#Set up mold by ensuring that the mold is securely assembled and is leak proof. | |||
#Place comb in mold. | |||
#Pour agar liquid gel in the flask into mold. | |||
#Wait for gel to set. | |||
#Remove gel from mold carefully without removing the comb. Take care not to tear the gel. | |||
#Transfer gel to electrophoresis apparatus. | |||
#Fill both sides of the electrophoresis apparatus with 1xTAE to the indicated line. | |||
#Then remove comb. | |||
#Using a micropipette, add 10 | |||
<u></u><br> | |||
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Revision as of 23:43, 7 July 2013
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Restriction Digest
ElectrophoresisPerparation of Gel
Preparation of Gel Plates
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