Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/24: Difference between revisions
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(Autocreate 2013/07/24 Entry for Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi) |
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== | ==Transformation of DH5α with KAH013 07/24/13== | ||
#LB plates were taken from fridge and placed in incubator at 37degrees. (By mistake LB-amp plates were selected instead of LB+amp) | |||
#KAH013 plasmid was taken from the fridge. | |||
#In a 1mL tube, 9μL of dH2O and 1μL of KAH013 were added. | |||
#DH5α cells were taken from the freezer and placed in an ice bath. | |||
#Waited 10 mins for the DH5α cells to thaw. | |||
#In the 1mL tube containing the dH2O and the plasmid, 50μL of DH5α was added. | |||
#Waited 10 mins for the bacteria to accept the plasmid. | |||
#Pipetted the 60μL contents of the 1mL tube onto the warmed LB-amp plates. | |||
#Around 6-10 rolling beads were used to spread the bacteria. | |||
#Plates were placed in incubator at 37degrees for 12 hours. | |||
Revision as of 14:52, 4 August 2013
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Transformation of DH5α with KAH013 07/24/13
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