Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/27

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(Incubation in Liquid Media)
(Incubation in Liquid Media)
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==Incubation in Liquid Media==
==Incubation in Liquid Media==
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1. Gathered 3 culture tubes, the three plates(mine, David's, and Heather's), LB+amp broth, a pipetter and a micropipette.
+
#Gathered 3 culture tubes, the three plates(mine, David's, and Heather's), LB+amp broth, a pipetter and a micropipette.
*Some parts/patches of the plates were dry.  
*Some parts/patches of the plates were dry.  
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2. Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids.  
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# Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids.  
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3. Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to my culture tube.  
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# Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to my culture tube.  
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4. Circled the colony I selected on my plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into my culture tube.
+
# Circled the colony I selected on my plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into my culture tube.
*Made sure micropipette tip did not touch the  
*Made sure micropipette tip did not touch the  
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5. Followed the same procedure with David's and Heather's culture tubes and plates.  
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# Followed the same procedure with David's and Heather's culture tubes and plates.  
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6. Placed all three tubes into the incubator.
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# Placed all three tubes into the incubator.
==Notes==
==Notes==

Revision as of 18:34, 27 July 2013

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Incubation in Liquid Media

  1. Gathered 3 culture tubes, the three plates(mine, David's, and Heather's), LB+amp broth, a pipetter and a micropipette.
  • Some parts/patches of the plates were dry.
  1. Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids.
  2. Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to my culture tube.
  3. Circled the colony I selected on my plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into my culture tube.
  • Made sure micropipette tip did not touch the
  1. Followed the same procedure with David's and Heather's culture tubes and plates.
  2. Placed all three tubes into the incubator.

Notes

NOTE: Plates were NOT wrapped in parafilm, so colonies may have been dried/dead when they were chosen

Plates were placed in the silver fridge on top of the blue plastic lid.


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