Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/27

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Incubation in Liquid Media

  1. Gathered 3 culture tubes, the three plates(mine, David's, and Heather's), LB+amp broth, a pipetter and a micropipette.
  • Some parts/patches of the plates were dry.
  1. Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids.
  2. Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to my culture tube.
  3. Circled the colony I selected on my plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into my culture tube.
  • Made sure micropipette tip did not touch the
  1. Followed the same procedure with David's and Heather's culture tubes and plates.
  2. Placed all three tubes into the incubator.


NOTE: Plates were NOT wrapped in parafilm, so colonies may have been dried/dead when they were chosen

Plates were placed in the silver fridge on top of the blue plastic lid.

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