Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/27: Difference between revisions
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==Incubation in Liquid Media== | ==Incubation in Liquid Media== | ||
#Gathered 3 culture tubes, the three plates(mine, David's, and Heather's), LB+amp broth, a pipetter and a micropipette. | |||
*Some parts/patches of the plates were dry. | *Some parts/patches of the plates were dry. | ||
# Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids. | |||
# Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to my culture tube. | |||
# Circled the colony I selected on my plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into my culture tube. | |||
*Made sure micropipette tip did not touch the | *Made sure micropipette tip did not touch the | ||
# Followed the same procedure with David's and Heather's culture tubes and plates. | |||
# Placed all three tubes into the incubator. | |||
==Notes== | ==Notes== |
Revision as of 15:34, 27 July 2013
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Incubation in Liquid Media
NotesNOTE: Plates were NOT wrapped in parafilm, so colonies may have been dried/dead when they were chosen Plates were placed in the silver fridge on top of the blue plastic lid. |