Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/27: Difference between revisions

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==Incubation in Liquid Media==
==Incubation in Liquid Media 7/27/13==
#Gathered 3 culture tubes, the three plates(mine, David's, and Heather's), LB+amp broth, a pipetter and a micropipette.
#Gathered 3 culture tubes, the three plates(Prad(mine), David's, and Heather's), LB+amp broth, a pipetter and a micropipette.
'''Some parts/patches of the plates were dry.'''
# Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids (Prad-KAH013, David-KAH06, Heather- also KAH013).  
# Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids.  
# Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to culture tube.  
# Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to my culture tube.  
# Circled the selected colony on the plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into the culture tube.
# Circled the colony I selected on my plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into my culture tube.
*Made sure micropipette tip did not touch the
# Followed the same procedure with David's and Heather's culture tubes and plates.  
# Followed the same procedure with David's and Heather's culture tubes and plates.  
# Placed all three tubes into the incubator.
# Placed all three tubes into the incubator.
*Plates were wrapped in parafilm and placed inside the silver fridge.


==Notes==
==Notes==
'''NOTE: Plates were NOT wrapped in parafilm, so colonies may have been dried/dead when they were chosen'''
'''NOTE: Plates were NOT wrapped in parafilm when I first saw them, and what appeared to be dry patches were on all 3 plates.'''


Plates were placed in the silver fridge on top of the blue plastic lid.  
Plates were placed in the silver fridge on top of the blue plastic lid.  

Revision as of 23:16, 2 December 2013

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Incubation in Liquid Media 7/27/13

  1. Gathered 3 culture tubes, the three plates(Prad(mine), David's, and Heather's), LB+amp broth, a pipetter and a micropipette.
  2. Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids (Prad-KAH013, David-KAH06, Heather- also KAH013).
  3. Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to culture tube.
  4. Circled the selected colony on the plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into the culture tube.
  5. Followed the same procedure with David's and Heather's culture tubes and plates.
  6. Placed all three tubes into the incubator.
  • Plates were wrapped in parafilm and placed inside the silver fridge.

Notes

NOTE: Plates were NOT wrapped in parafilm when I first saw them, and what appeared to be dry patches were on all 3 plates.

Plates were placed in the silver fridge on top of the blue plastic lid.


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