Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/07/27: Difference between revisions
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==Incubation in Liquid Media 7/27/13== | ==Incubation in Liquid Media 7/27/13== | ||
#Gathered 3 culture tubes, the three plates(mine, David's, and Heather's), LB+amp broth, a pipetter and a micropipette. | #Gathered 3 culture tubes, the three plates(Prad(mine), David's, and Heather's), LB+amp broth, a pipetter and a micropipette. | ||
# Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids ( | # Labled each tube with LB+amp, each person's initials on their respective culture tubes, and plasmids (Prad-KAH013, David-KAH06, Heather- also KAH013). | ||
# Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to | # Used pipetter and the 5 mL pipette tip to transfer 3mL of LB+amp broth to culture tube. | ||
# Circled the colony | # Circled the selected colony on the plate and then used the micropipette to lightly touch the colony and ejected the micropipette tip into the culture tube. | ||
# Followed the same procedure with David's and Heather's culture tubes and plates. | # Followed the same procedure with David's and Heather's culture tubes and plates. | ||
# Placed all three tubes into the incubator. | # Placed all three tubes into the incubator. |
Revision as of 23:13, 2 December 2013
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Incubation in Liquid Media 7/27/13
NotesNOTE: Plates were NOT wrapped in parafilm when I first saw them, so colonies may have been dried/dead when they were chosen Plates were placed in the silver fridge on top of the blue plastic lid. |