Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/09/21: Difference between revisions

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==09/21/13==
==09/21/13==
*Growth on streak plate


*'''AmCyan and flyPCD'''
**Inoculated: [http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/09/20 9/20/13]
**Culture 1(AmCyan): Growth
**Culture 2(flyPCD): Growth


==Samples For Miniprep and Analysis==
Sample 1: Colony 1, BBa_J176022, part = 687, vector = 4968
Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968


==List of Samples==
==List of Samples==
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| Plasmid || OD260 || OD260/280 || ng/μL
| Plasmid || OD260 || OD260/280 || ng/μL
|-
|-
| 1. Plasmid 1 || .027 || 1.971 || 26.921
| 1. AmCyan || 0.034 || 1.8 || 34.227
|-
|-
| 2. Plasmid 2 || .03 || 1.895 || 29.764
| 2. flyPCD || 0.025 || 1.953 || 24.576
|}
|}


'''Restriction Digest Table'''<br>
'''Restriction Digest Table'''<br>
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| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Reagent  
| bgcolor=#cfcfcf | Volume
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 AmCyan = 687bp,4968bp<br>2. Plasmid 2 flyPCD= 186bp, 4968 bp<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
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|}
|}


*'''RESTRICTION DIGEST yielded blank gel, miniprep yields may have been too low. Cultures will be inoculated again, and miniprep will be performed again'''


'''DNA Sequencing Samples'''
'''DNA Sequencing Samples'''

Revision as of 14:31, 14 October 2013

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09/21/13

List of Samples

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. AmCyan 0.034 1.8 34.227
2. flyPCD 0.025 1.953 24.576

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 AmCyan = 687bp,4968bp
2. Plasmid 2 flyPCD= 186bp, 4968 bp
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.
  • RESTRICTION DIGEST yielded blank gel, miniprep yields may have been too low. Cultures will be inoculated again, and miniprep will be performed again

DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'




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