Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/09/21

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(List of Samples)
(List of Samples)
Line 32: Line 32:
| Plasmid || OD260 || OD260/280 || ng/μL
| Plasmid || OD260 || OD260/280 || ng/μL
|-
|-
-
| 1. AmCyan || .027 || 1.971 || 26.921
+
| 1. AmCyan || 0.034 || 1.8 || 34.227
|-
|-
-
| 2. flyPCD || .03 || 1.895 || 29.764
+
| 2. flyPCD || 0.025 || 1.953 || 24.576
|}
|}
-
 
'''Restriction Digest Table'''<br>
'''Restriction Digest Table'''<br>

Revision as of 17:57, 21 September 2013

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09/21/13

  • Growth on streak plate
  • AmCyan and flyPCD
    • Inoculated: 9/20/13
    • Culture 1(AmCyan): Growth
    • Culture 2(flyPCD): Growth

Samples For Miniprep and Analysis

Sample 1: Colony 1, BBa_J176022, part = 687, vector = 4968 Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968

List of Samples

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. AmCyan 0.034 1.8 34.227
2. flyPCD 0.025 1.953 24.576

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'




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