Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/10/14

From OpenWetWare

< Haynes Lab:Notebook | Lab Traning - Pradyumna Kadambi | 2013 | 10(Difference between revisions)
Jump to: navigation, search
(List of Samples)
Current revision (01:52, 3 December 2013) (view source)
(List of Samples)
 
(4 intermediate revisions not shown.)
Line 47: Line 47:
| bgcolor=#cfcfcf | Volume
| bgcolor=#cfcfcf | Volume
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 AmCyan = 687bp,4968bp<br>2. Plasmid 2 flyPCD= 186bp, 4968 bp<br>
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 AmCyan = 687bp,4968bp<br>2. Plasmid 2 flyPCD= 186bp, 4968 bp<br>
-
| rowspan="7" | [[Image:Gel_Results_Bootcamp.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
+
| rowspan="7" | [[Image:Gel_Results_Cloning.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
| DNA(plasmid) || 3.0 μL
| DNA(plasmid) || 3.0 μL
Line 62: Line 62:
|}
|}
 +
Faint lines at roughly 200bp were observed for wells 1-3 consistent with the expected results for flyPCD, and faint lines were visible at roughly 700bp for wells 4-6 consistent with the expected results for AmCyan. Also strong lines were observed at around 4000-5000 for all wells 1-6, which match the vector backbone. 
'''DNA Sequencing Samples'''
'''DNA Sequencing Samples'''
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples -->
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples -->
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below -->
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below -->
-
* Submitted to DNASU on: '10/16/2013'
+
* Submitted to DNASU on: '11/14/2013'
-
* Order Number:  
+
* Order Number: 8388
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
# Plasmid 1 AmCyan - forward primer 'P001'
# Plasmid 1 AmCyan - forward primer 'P001'

Current revision

Project name Main project page
Previous entry      

10/14/13

Good expected growth on streak plate, and good growth in both liquid cultures.

Samples For Miniprep and Analysis

Sample 1: Colony 1, BBa_J176022, part = 687, vector = 4968 Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968

List of Samples

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. AmCyan 0.161 1.85 160.992
2. flyPCD 0.358 1.818 358.374

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 AmCyan = 687bp,4968bp
2. Plasmid 2 flyPCD= 186bp, 4968 bp
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.

Faint lines at roughly 200bp were observed for wells 1-3 consistent with the expected results for flyPCD, and faint lines were visible at roughly 700bp for wells 4-6 consistent with the expected results for AmCyan. Also strong lines were observed at around 4000-5000 for all wells 1-6, which match the vector backbone.

DNA Sequencing Samples

  • Submitted to DNASU on: '11/14/2013'
  • Order Number: 8388
  1. Plasmid 1 AmCyan - forward primer 'P001'
  2. Plasmid 1 AmCyan - reverse primer 'P002'
  3. Plasmid 2 flyPCD - forward primer 'P001'
  4. Plasmid 2 flyPCD - reverse primer 'P002'




Personal tools