Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/10/14: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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| bgcolor=#cfcfcf | Volume
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| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 AmCyan = 687bp,4968bp<br>2. Plasmid 2 flyPCD= 186bp, 4968 bp<br>
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 AmCyan = 687bp,4968bp<br>2. Plasmid 2 flyPCD= 186bp, 4968 bp<br>
| rowspan="7" | [[Image:Gel_Results_Bootcamp.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="7" | [[Image:Gel_Results_Cloning.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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| DNA(plasmid) || 3.0 μL
| DNA(plasmid) || 3.0 μL
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Faint lines at roughly 200bp were observed for wells 1-3 consistent with the expected results for flyPCD, and faint lines were visible at roughly 700bp for wells 4-6 consistent with the expected results for AmCyan. Also strong lines were observed at around 4000-5000 for all wells 1-6, which match the vector backbone. 


'''DNA Sequencing Samples'''
'''DNA Sequencing Samples'''
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples -->
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples -->
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below -->
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below -->
* Submitted to DNASU on: '10/16/2013'
* Submitted to DNASU on: '11/14/2013'
* Order Number:  
* Order Number: 8388
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
# Plasmid 1 AmCyan - forward primer 'P001'
# Plasmid 1 AmCyan - forward primer 'P001'

Latest revision as of 23:27, 26 September 2017

Project name Main project page
Previous entry      

10/14/13

Good expected growth on streak plate, and good growth in both liquid cultures.

Samples For Miniprep and Analysis

Sample 1: Colony 1, BBa_J176022, part = 687, vector = 4968 Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968

List of Samples

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. AmCyan 0.161 1.85 160.992
2. flyPCD 0.358 1.818 358.374

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 AmCyan = 687bp,4968bp
2. Plasmid 2 flyPCD= 186bp, 4968 bp
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.

Faint lines at roughly 200bp were observed for wells 1-3 consistent with the expected results for flyPCD, and faint lines were visible at roughly 700bp for wells 4-6 consistent with the expected results for AmCyan. Also strong lines were observed at around 4000-5000 for all wells 1-6, which match the vector backbone.

DNA Sequencing Samples

  • Submitted to DNASU on: '11/14/2013'
  • Order Number: 8388
  1. Plasmid 1 AmCyan - forward primer 'P001'
  2. Plasmid 1 AmCyan - reverse primer 'P002'
  3. Plasmid 2 flyPCD - forward primer 'P001'
  4. Plasmid 2 flyPCD - reverse primer 'P002'




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