Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/10/14: Difference between revisions
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| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 AmCyan = 687bp,4968bp<br>2. Plasmid 2 flyPCD= 186bp, 4968 bp<br> | | rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 AmCyan = 687bp,4968bp<br>2. Plasmid 2 flyPCD= 186bp, 4968 bp<br> | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:Gel_Results_Cloning.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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| DNA(plasmid) || 3.0 μL | | DNA(plasmid) || 3.0 μL | ||
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Faint lines at roughly 200bp were observed for wells 1-3 consistent with the expected results for flyPCD, and faint lines were visible at roughly 700bp for wells 4-6 consistent with the expected results for AmCyan. Also strong lines were observed at around 4000-5000 for all wells 1-6, which match the vector backbone. | |||
'''DNA Sequencing Samples''' | '''DNA Sequencing Samples''' | ||
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | <!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | ||
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below --> | <!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below --> | ||
* Submitted to DNASU on: ' | * Submitted to DNASU on: '11/14/2013' | ||
* Order Number: | * Order Number: 8388 | ||
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | <!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | ||
# Plasmid 1 - forward primer ' | # Plasmid 1 AmCyan - forward primer 'P001' | ||
# Plasmid 1 - reverse primer ' | # Plasmid 1 AmCyan - reverse primer 'P002' | ||
# Plasmid 2 - forward primer ' | # Plasmid 2 flyPCD - forward primer 'P001' | ||
# Plasmid 2 - reverse primer ' | # Plasmid 2 flyPCD - reverse primer 'P002' | ||
Revision as of 22:52, 2 December 2013
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> | ||||||||||||||||||||||||||||
10/14/13Good expected growth on streak plate, and good growth in both liquid cultures. Samples For Miniprep and AnalysisSample 1: Colony 1, BBa_J176022, part = 687, vector = 4968 Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968 List of SamplesDNA Concentration Data
Restriction Digest Table
Faint lines at roughly 200bp were observed for wells 1-3 consistent with the expected results for flyPCD, and faint lines were visible at roughly 700bp for wells 4-6 consistent with the expected results for AmCyan. Also strong lines were observed at around 4000-5000 for all wells 1-6, which match the vector backbone. DNA Sequencing Samples
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