Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/10/14

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(Autocreate 2013/10/14 Entry for Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi)
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==Entry title==
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==10/14/13==
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==Samples For Miniprep and Analysis==
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Sample 1: Colony 1, BBa_J176022, part = 687, vector = 4968
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Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968
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==List of Samples==
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'''DNA Concentration Data'''
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|- bgcolor=#cfcfcf
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| Plasmid || OD260 || OD260/280 || ng/μL
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| 1. AmCyan || 0.034 || 1.8 || 34.227
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| 2. flyPCD || 0.025 || 1.953 || 24.576
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|}
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'''Restriction Digest Table'''<br>
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* Checked plasmid minipreps with EcoRI/PstI digests
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{| {{table}} border="1" cellspacing="3"
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<!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2
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|- valign="top"
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| bgcolor=#cfcfcf | Reagent
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| bgcolor=#cfcfcf | Volume
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| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 AmCyan = 687bp,4968bp<br>2. Plasmid 2 flyPCD= 186bp, 4968 bp<br>
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| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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|-
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| DNA(plasmid) || 3.0 μL
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| 10X buffer || 1.5
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| EcoRI || 1.0
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|-
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| PstI || 1.0
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|-
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| dH<sub>2</sub>O || 8.5
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| &nbsp; || 15 μL --> 37°C/ 15 min.
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|}
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*'''RESTRICTION DIGEST yielded blank gel, miniprep yields may have been too low. Cultures will be inoculated again, and miniprep will be performed again'''
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'''DNA Sequencing Samples'''
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* Submitted to DNASU on: 'date'
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* Order Number:
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<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
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# Plasmid 1 - forward primer 'name'
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# Plasmid 1 - reverse primer 'name'
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# Plasmid 2 - forward primer 'name'
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# Plasmid 2 - reverse primer 'name'
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Revision as of 17:32, 14 October 2013

Project name Main project page
Previous entry      

10/14/13

Samples For Miniprep and Analysis

Sample 1: Colony 1, BBa_J176022, part = 687, vector = 4968 Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968

List of Samples

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. AmCyan 0.034 1.8 34.227
2. flyPCD 0.025 1.953 24.576

Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 AmCyan = 687bp,4968bp
2. Plasmid 2 flyPCD= 186bp, 4968 bp
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.
  • RESTRICTION DIGEST yielded blank gel, miniprep yields may have been too low. Cultures will be inoculated again, and miniprep will be performed again

DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'




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