Haynes Lab:Notebook/Protein-DNA fusions in living microbes/2013/09/21

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(Autocreate 2013/09/21 Entry for Haynes_Lab:Notebook/Protein-DNA_fusions_in_living_microbes)
Current revision (02:54, 3 December 2013) (view source)
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==Entry title==
==Entry title==
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-Ran topoisomerase reaction titration:
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pSB1C3 2ug 2ug 2ug 2ug 2ug
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topo 2uL 1.5uL 1uL 0.5uL 1.5uL 50% glycerol
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NEB 4 3uL 3uL 3uL 3uL 3uL
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ddH20 up to 30
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total 30uL 30uL 30uL 30uL 30uL
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incubated at 37C for 10 minutes, then transferred to -20C to stop reaction
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-Grew up liquid Kan cultures of 2 colonies containing TopoD168A pET29a

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-Ran topoisomerase reaction titration: pSB1C3 2ug 2ug 2ug 2ug 2ug topo 2uL 1.5uL 1uL 0.5uL 1.5uL 50% glycerol NEB 4 3uL 3uL 3uL 3uL 3uL ddH20 up to 30 total 30uL 30uL 30uL 30uL 30uL

incubated at 37C for 10 minutes, then transferred to -20C to stop reaction

-Grew up liquid Kan cultures of 2 colonies containing TopoD168A pET29a



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