Haynes Lab:Notebook/Protein-DNA fusions in living microbes/2013/10/09: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav)
 
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|

Latest revision as of 23:36, 26 September 2017

Project name Main project page
Previous entry      Next entry

Entry title

Performed successful tranformation with Kylie's BL21(DE3) used protocol here: http://www.molecularinfo.com/MTM/A/A3/A3-1.pdf

Made SOC media: LB miler broth + tryptone + 20 mM MgSO4 and 2.5 mM KCl (http://openwetware.org/wiki/SOB)

Grew up 4 colonies of BL21(DE3) in Kan media, used 3 of them (1, 2, 4) to induce expression of Topo per protocol: http://www.molecularinfo.com/MTM/A/A3/A3-1.pdf

Ran PCR using 1-omega F TSE and alpha-4 R SP and PSV plasmid template. PCR product has 3 topo cut sites.

Ran gel of wheat-germ topo +RFP pSB1C3 plasmid. From left to right: 2uL, 1.5uL, 1uL, 0.5uL, blank glycerol stock (15/30 uL in each well)

Made competent cell stock of BL21(DE3) from cells given by Kylie

Used Bug buster on induced samples (1, 2, 4) per protocol: http://www.ebiotrade.com/buyf/productsf/novagen/70584-000.pdf


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Protein-DNA_fusions_in_living_microbes/2013/10/09&oldid=1013818"