Haynes Lab:Notebook/Protein-DNA fusions in living microbes/2013/10/24: Difference between revisions
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Ryan Muller (talk | contribs) (Autocreate 2013/10/24 Entry for Haynes_Lab:Notebook/Protein-DNA_fusions_in_living_microbes) |
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==Entry title== | ==Entry title== | ||
pSB1C3 500ng 500ng 500ng 500ng 500ng 500ng | |||
14.7uL 14.7uL 14.7uL 14.7uL 14.7uL 14.7uL | |||
topo eq. 0.5uL 0.4uL 0.3uL 0.2uL 0.1uL 0uL | |||
2.5uL 2uL 1.5uL 1uL 0.5uL 0uL | |||
50% glycerol 0 0.5uL 1uL 1.5uL 2uL 2.5uL | |||
NEB 4 3uL 3uL 3uL 3uL 3uL 3uL | |||
ddH20 up to 30 | |||
total 20uL 30uL 30uL 30uL 30uL 30uL | |||
incubated at 37C for 5 minutes, then transferred to -20C to stop reaction | |||
(loaded 7 uL of ladder) | |||
3 topo site PCR product: 1uL | |||
Neb 4 buffer: 3uL (accidentally added twice as much) | |||
Topo induced or uninduced: 1uL | |||
water 11uL | |||
total 16uL | |||
Revision as of 00:03, 3 December 2013
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Entry titlepSB1C3 500ng 500ng 500ng 500ng 500ng 500ng 14.7uL 14.7uL 14.7uL 14.7uL 14.7uL 14.7uL topo eq. 0.5uL 0.4uL 0.3uL 0.2uL 0.1uL 0uL 2.5uL 2uL 1.5uL 1uL 0.5uL 0uL 50% glycerol 0 0.5uL 1uL 1.5uL 2uL 2.5uL NEB 4 3uL 3uL 3uL 3uL 3uL 3uL ddH20 up to 30 total 20uL 30uL 30uL 30uL 30uL 30uL incubated at 37C for 5 minutes, then transferred to -20C to stop reaction (loaded 7 uL of ladder) 3 topo site PCR product: 1uL Neb 4 buffer: 3uL (accidentally added twice as much) Topo induced or uninduced: 1uL water 11uL total 16uL
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