Haynes Lab:Notebook/Protein-DNA fusions in living microbes/2013/10/24

From OpenWetWare

< Haynes Lab:Notebook | Protein-DNA fusions in living microbes | 2013 | 10
Revision as of 03:03, 3 December 2013 by Ryan Muller (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Entry title

pSB1C3 500ng 500ng 500ng 500ng 500ng 500ng 14.7uL 14.7uL 14.7uL 14.7uL 14.7uL 14.7uL topo eq. 0.5uL 0.4uL 0.3uL 0.2uL 0.1uL 0uL 2.5uL 2uL 1.5uL 1uL 0.5uL 0uL 50% glycerol 0 0.5uL 1uL 1.5uL 2uL 2.5uL NEB 4 3uL 3uL 3uL 3uL 3uL 3uL ddH20 up to 30 total 20uL 30uL 30uL 30uL 30uL 30uL incubated at 37C for 5 minutes, then transferred to -20C to stop reaction (loaded 7 uL of ladder)

3 topo site PCR product: 1uL Neb 4 buffer: 3uL (accidentally added twice as much) Topo induced or uninduced: 1uL water 11uL total 16uL

Personal tools