Haynes Lab:Notebook/Protein-DNA fusions in living microbes/2013/11/09: Difference between revisions

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==Entry title==
==Entry title==
* Insert content here...
Reran gel of 9/21 experiment
 
Ran time course:
pSB1C3 500ng 500ng 500ng 500ng 500ng 500ng
19.2uL 19.2uL 19.2uL 19.2uL 19.2uL 19.2uL
topo eq. 0.5uL 0.5uL 0.5uL 0.5uL 0.5uL 1uL EcoRI
NEB 4 3uL 3uL 3uL 3uL 3uL 3uL
ddH20 up to 30
total 30uL 30uL 30uL 30uL 30uL 30uL
incubation:37C 4min 6 8 10 12 12+




Latest revision as of 23:37, 26 September 2017

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Reran gel of 9/21 experiment

Ran time course: pSB1C3 500ng 500ng 500ng 500ng 500ng 500ng 19.2uL 19.2uL 19.2uL 19.2uL 19.2uL 19.2uL topo eq. 0.5uL 0.5uL 0.5uL 0.5uL 0.5uL 1uL EcoRI

NEB 4 3uL 3uL 3uL 3uL 3uL 3uL ddH20 up to 30 total 30uL 30uL 30uL 30uL 30uL 30uL incubation:37C 4min 6 8 10 12 12+



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