Haynes Lab:Notebook/Protein-DNA fusions in living microbes/2013/11/21: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/11/21 Entry for Haynes_Lab:Notebook/Protein-DNA_fusions_in_living_microbes)
 
(fix raw html notebook nav)
 
(One intermediate revision by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Entry title==
* Insert content here...
testing use of NH4SO4 to halt topoisomerase reaction.  
 
pSB1C3 250ng 250ng 250ng
7.35uL 7.35uL 7.35uL
topo eq. 0.5uL 0.5uL 0.5uL
stop mechanism 4M am.sulfamate 4M am.acetate 0M ctrl
16M stock: 15uL 15uL 0
NEB 4 3uL 3uL 3uL
ddH20 up to 30
total 30uL 30uL 30uL
incubation:37C 10min 10 10
 
ammonium acetate: 12.32g/10mL
ammonium sulfamate: 18.26g/10mL
 
stop mechanism (ammonium sulfamate and ammonium acetate) added before incubation.
incubate at 37 for 10 minutes
place in -20 C
run on gel




Latest revision as of 23:37, 26 September 2017

Project name Main project page
Previous entry      

Entry title

testing use of NH4SO4 to halt topoisomerase reaction.

pSB1C3 250ng 250ng 250ng 7.35uL 7.35uL 7.35uL topo eq. 0.5uL 0.5uL 0.5uL stop mechanism 4M am.sulfamate 4M am.acetate 0M ctrl 16M stock: 15uL 15uL 0 NEB 4 3uL 3uL 3uL ddH20 up to 30 total 30uL 30uL 30uL incubation:37C 10min 10 10

ammonium acetate: 12.32g/10mL ammonium sulfamate: 18.26g/10mL

stop mechanism (ammonium sulfamate and ammonium acetate) added before incubation. incubate at 37 for 10 minutes place in -20 C run on gel



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Protein-DNA_fusions_in_living_microbes/2013/11/21&oldid=1013824"