Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/19: Difference between revisions
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==Results/Conclusions== | ==Results/Conclusions== | ||
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Revision as of 10:41, 19 December 2014
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SummaryNOTE: Today's procedure is a repeat of the experiment on 12/16/2014, using a higher-concentration plasmid DNA source. In order to introduce a new gene into the mammalian cancer cells, we need to make the cells competent and present them with a prepared plasmid containing our gene of interest along with a selectable marker. Lipofectamine is a tranfection reagent that produces positively charged liposomes that envelop the plasmids to be introduced to the cells and encourage passage through the negatively charged cell membrane into the cells. Goals
Protocolmammalian cell transfection with liopfectamine Materials
NotesTo get your plasmid to the appropriate concentration, add 2.0μg to each 1.5mL microcentrifuge tube and then add nuclease-free water to bring the total volume to 20μL.
Results/Conclusions |