Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Goals== | ==Goals== | ||
* | * Passage U2OS cells from PS-2 to PS-3<br><br> | ||
==Protocol== | ==Protocol== | ||
[http://openwetware.org/wiki/Haynes: | [http://openwetware.org/wiki/Haynes:SplittingCells splitting cells]<br><br> | ||
==Materials== | ==Materials== | ||
* | * 70% ethanol spray bottle | ||
* | * 1x Phosphate-buffered saline (PBS) | ||
* Trypsin-EDTA buffer/ media | |||
* Complete cell culture medium (e.g., 10% FBS, 1% pen-strep) | |||
* Sterile tissue culture-treated vent-capped T-75 flask, one per new culture | |||
* Pipette tips, etc. | |||
* 100% confluent (dense) cell culture (check confluency under the light microscope) | |||
==Notes== | ==Notes== | ||
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==Results/Conclusions== | ==Results/Conclusions== | ||
U2OS cells in passage PS-3 are in a T-75 flask in the upper incubator, third shelf from the top.<br><br> | |||
*'''[[User:David Benjamin Nyer|David Benjamin Nyer]] | *'''[[User:David Benjamin Nyer|David Benjamin Nyer]] 15:18, 24 December 2014 (EST)''': | ||
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__NOTOC__ | __NOTOC__ |
Latest revision as of 00:36, 27 September 2017
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | Main project page Previous entry Next entry |
SummaryThe second transfection attempt is also showing extremely low levels of PcTF-mCherry protein in the U2OS cell line (fluorescence microscopy images not shown here). Today being Christmas Eve, I'm going to wait until next week before making another attempt. Goals
ProtocolMaterials
NotesObservations, measurements, placeholder info etc. go here Results/ConclusionsU2OS cells in passage PS-3 are in a T-75 flask in the upper incubator, third shelf from the top.
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