Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/23: Difference between revisions
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==Summary== | ==Summary== | ||
The KAH126/U2OS cells in the 6-well plate that were induced to produce PcTF-RFP back on Monday are ready for the first round of harvesting. I'll be isolating the RNA from them and storing the RNA at -80°C before sending the samples out for analysis early next week. | |||
Also need to passage the SKNSH culture from PS-β to PS-1 and the KAH126/U2OS culture from PS-4 to PS-5. | |||
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Revision as of 16:46, 23 January 2015
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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SummaryThe KAH126/U2OS cells in the 6-well plate that were induced to produce PcTF-RFP back on Monday are ready for the first round of harvesting. I'll be isolating the RNA from them and storing the RNA at -80°C before sending the samples out for analysis early next week. Also need to passage the SKNSH culture from PS-β to PS-1 and the KAH126/U2OS culture from PS-4 to PS-5.
Goals
Protocol
NotesObservations, measurements, placeholder info etc. go here.
Results/ConclusionsData collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.
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