Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/23: Difference between revisions

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==Results/Conclusions==
==Results/Conclusions==
Data collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.
 
Photographs of the KAH126/U2OS samples induced on 1/19 (4 days post-induction):
 
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_1_phase_%26_mCherry_10x.jpg | 250px | Well 1]]
 
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_2_phase_%26_mCherry_10x.jpg | 250px | Well 2]]
 
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_3_phase_%26_mCherry_10x.jpg | 250px | Well 3]]
 
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_4_phase_%26_mCherry_10x.jpg | 250px | Well 4]]
 
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_5_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 5 (negative control)]]
 
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_6_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 6 (negative control)]]
 
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Photographs of the KAH126/U2OS samples induced on 1/22 (1 day post-induction):
 
[[Image:2015-01-23_KAH126-U2OS_day1postinduction_Well_1_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 1 (negative control)]]
 
[[Image:2015-01-23_KAH126-U2OS_day1postinduction_Well_2_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 2 (negative control)]]
 
[[Image:2015-01-23_KAH126-U2OS_day1postinduction_Well_3_phase_%26_mCherry_10x.jpg | 250px | Well 3]]
 
[[Image:2015-01-23_KAH126-U2OS_day1postinduction_Well_4_phase_%26_mCherry_10x.jpg | 250px | Well 4]]
 
[[Image:2015-01-23_KAH126-U2OS_day1postinduction_Well_5_phase_%26_mCherry_10x.jpg | 250px | Well 5]]
 
[[Image:2015-01-23_KAH126-U2OS_day1postinduction_Well_6_phase_%26_mCherry_10x.jpg | 250px | Well 6]]
 
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Photographs of the U2OS samples transfected on 1/22 (1 day post-transfection):
 
[[Image:2015-01-23_U2OS_1dayposttransfection_Well_1_phase_%26_mCherry_10x.jpg | 250px | Well 1]]
 
[[Image:2015-01-23_U2OS_1dayposttransfection_Well_2_phase_%26_mCherry_10x.jpg | 250px | Well 2]]
 
[[Image:2015-01-23_U2OS_1dayposttransfection_Well_3_phase_%26_mCherry_10x.jpg | 250px | Well 3]]
 
[[Image:2015-01-23_U2OS_1dayposttransfection_Well_4_phase_%26_mCherry_10x.jpg | 250px | Well 4]]
 
[[Image:2015-01-23_U2OS_1dayposttransfection_Well_5_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 5 (negative control)]]
 
[[Image:2015-01-23_U2OS_1dayposttransfection_Well_6_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 6 (negative control)]]




Revision as of 16:58, 23 January 2015

RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

The KAH126/U2OS cells in the 6-well plate that were induced to produce PcTF-RFP back on Monday are ready for the first round of harvesting. I'll be isolating the RNA from them and storing the RNA at -80°C before sending the samples out for analysis early next week.

Also need to passage the SKNSH culture from PS-β to PS-1 and the KAH126/U2OS culture from PS-4 to PS-5.



Goals

  • Passage SKNSH
  • Passage KAH126/U2OS, save some for Karmella's plates
  • Harvest RNA from KAH126/U2OS induced on Jan 19th



Protocol

TRIzol/RNEasy Spin Column Protocol

passaging mammalian cells




Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

Photographs of the KAH126/U2OS samples induced on 1/19 (4 days post-induction):

Well 1

Well 2

Well 3

Well 4

Well 5 (negative control)

Well 6 (negative control)

Photographs of the KAH126/U2OS samples induced on 1/22 (1 day post-induction):

Well 1 (negative control)

Well 2 (negative control)

Well 3

Well 4

Well 5

Well 6

Photographs of the U2OS samples transfected on 1/22 (1 day post-transfection):

Well 1

Well 2

Well 3

Well 4

Well 5 (negative control)

Well 6 (negative control)



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