Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/23: Difference between revisions
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Photographs of the KAH126/U2OS samples induced on 1/19 (4 days post-induction): | Photographs of the KAH126/U2OS samples induced on 1/19 (4 days post-induction): | ||
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_1_phase_%26_mCherry_10x.jpg | 250px | Well 1]] | [[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_1_phase_%26_mCherry_10x.jpg | 250px | Well 1]] [[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_2_phase_%26_mCherry_10x.jpg | 250px | Well 2]] | ||
[[Image:2015-01-23_KAH126- | [[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_3_phase_%26_mCherry_10x.jpg | 250px | Well 3]] [[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_4_phase_%26_mCherry_10x.jpg | 250px | Well 4]] | ||
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_5_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 5 (negative control)]] [[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_6_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 6 (negative control)]] | |||
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_5_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 5 (negative control)]] | |||
[[Image:2015-01-23_KAH126-U2OS_day4postinduction_Well_6_negative_control_phase_%26_mCherry_10x.jpg | 250px | Well 6 (negative control)]] | |||
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Revision as of 16:59, 23 January 2015
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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SummaryThe KAH126/U2OS cells in the 6-well plate that were induced to produce PcTF-RFP back on Monday are ready for the first round of harvesting. I'll be isolating the RNA from them and storing the RNA at -80°C before sending the samples out for analysis early next week. Also need to passage the SKNSH culture from PS-β to PS-1 and the KAH126/U2OS culture from PS-4 to PS-5.
Goals
ProtocolTRIzol/RNEasy Spin Column Protocol
NotesObservations, measurements, placeholder info etc. go here.
Results/ConclusionsPhotographs of the KAH126/U2OS samples induced on 1/19 (4 days post-induction):
Photographs of the KAH126/U2OS samples induced on 1/22 (1 day post-induction):
Photographs of the U2OS samples transfected on 1/22 (1 day post-transfection):
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