Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/26: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span>
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Latest revision as of 00:47, 27 September 2017

RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Main project page
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Summary

In paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.



Goals

  • ✓ Measure K562 cell density via FC
  • ✓ Passage K562 cell line



Protocol

passaging cells

flow cytometry




Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

Cell density measured at 287,000 cells/mL. This is a 3-fold increase from yesterday's counts; at this rate, cells will be ready for transfection tomorrow.