Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/27: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Goals) |
(fix raw html notebook nav) |
||
(6 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 13: | Line 13: | ||
==Goals== | ==Goals== | ||
<!-- Precede finished items with a checkmark ✓ --> | <!-- Precede finished items with a checkmark ✓ --> | ||
* Measure cell density of K562 culture via flow cytometry | * ✓ Measure cell density of K562 culture via flow cytometry | ||
* If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid | * ✓ If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid | ||
<br><br> | <br><br> | ||
==Protocol== | ==Protocol== | ||
[http://openwetware.org/wiki/Haynes: | [http://openwetware.org/wiki/Haynes:FCMamalian_FluorGene flow cytometry] | ||
[http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo transfection with lipofectamine] | |||
<br><br> | <br><br> | ||
==Notes== | ==Notes== | ||
Well 1: KAH126/MV2, ben's plasmid prep (original concentration 377 ng/μL)<br> | |||
Well 2: KAH126/MV2, Mara's plasmid prep (original concentration 279 ng/μL)<br> | |||
Well 3: negative control, no plasmid | |||
<br><br> | <br><br> | ||
==Results/Conclusions== | ==Results/Conclusions== | ||
Culture density is 668,000 cells/mL as measured via flow cytometry. | |||
Cells have been transfected and placed in the incubator. Tomorrow: fluorescence microscopy to check transfection efficiency. | |||
Latest revision as of 00:47, 27 September 2017
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | Main project page Previous entry Next entry |
SummaryIn paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.
Goals
Protocoltransfection with lipofectamine
NotesWell 1: KAH126/MV2, ben's plasmid prep (original concentration 377 ng/μL)
Results/ConclusionsCulture density is 668,000 cells/mL as measured via flow cytometry. Cells have been transfected and placed in the incubator. Tomorrow: fluorescence microscopy to check transfection efficiency.
|