Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/27: Difference between revisions
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==Goals== | ==Goals== | ||
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* Measure cell density of K562 culture via flow cytometry | * ✓ Measure cell density of K562 culture via flow cytometry | ||
* If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid | * If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid | ||
Revision as of 14:40, 27 February 2015
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SummaryIn paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.
Goals
Protocoltransfection with lipofectamine
NotesWell 1: KAH126/MV2, ben's plasmid prep (original concentration 377 ng/μL)
Results/ConclusionsData collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.
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