Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/27: Difference between revisions
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* ✓ Measure cell density of K562 culture via flow cytometry | * ✓ Measure cell density of K562 culture via flow cytometry | ||
* If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid | * ✓ If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid | ||
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Culture density is 668,000 cells/mL as measured via flow cytometry. | Culture density is 668,000 cells/mL as measured via flow cytometry. | ||
Cells have been transfected and placed in the incubator. Tomorrow: fluorescence microscopy to check transfection efficiency. | |||
Revision as of 15:17, 27 February 2015
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SummaryIn paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.
Goals
Protocoltransfection with lipofectamine
NotesWell 1: KAH126/MV2, ben's plasmid prep (original concentration 377 ng/μL)
Results/ConclusionsCulture density is 668,000 cells/mL as measured via flow cytometry. Cells have been transfected and placed in the incubator. Tomorrow: fluorescence microscopy to check transfection efficiency.
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