Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/27

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RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

In paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.



Goals

  • Measure cell density of K562 culture via flow cytometry
  • If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid



Protocol

flow cytometry

transfection with lipofectamine




Notes

Well 1: KAH126/MV2, ben's plasmid prep (original concentration 377 ng/μL)
Well 2: KAH126/MV2, Mara's plasmid prep (original concentration 279 ng/μL)
Well 3: negative control, no plasmid



Results/Conclusions

Data collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.



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