Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/04/15: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 49: | Line 49: | ||
<b>--Gel Electrophoresis--</b> | <b>--Gel Electrophoresis--</b> | ||
[[Image:2015-04-15_KAH187-MV8_digest_annotated.png | [[Image:2015-04-15_KAH187-MV8_digest_annotated.png]] | ||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 18:21, 15 April 2015
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||
Plasmid Maxiprep of KAH187-MV8--Notes-- During the plasmid purification procedure, I accidentally mixed the lysate flowthrough (containing plasmid) with the column preparation solution that was run through the Binding Column. Proceeding as normal from this point forward. Hopefully doesn't influence yield. --DNA Quantification-- Measured using the Take3 plate:
--Restriction Enzyme Digest--
Digest for 15 minutes at 37°C. --Gel Electrophoresis--
|