Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/15
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Transfection of K562 using KAH187-MV8, KAH126-MV2, KAH87-MV10 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||
OverviewRepeating last week's experiment with K562 and a working copy of the KAH87-MV10 plasmid. Transfection SetupSee the Haynes Lab transfection protocol for details. One difference: final volume of antibiotic-free medium in each well is 2mL, not 4mL. All other values kept the same.
Cell density as measured by hemacytometer is 460,000 cells per well.
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