Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/21: Difference between revisions
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| pMaxGFP pre-concentration||486.333||7.966264997||1.888 | | pMaxGFP pre-concentration||486.333||7.966264997||1.888 | ||
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| evap conc.||2090. | | evap conc.||2090.9||17.05||1.90 | ||
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| evap conc, 10x dil||190. | | evap conc, 10x dil||190.1||1.86||1.91 | ||
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| precip conc.||2297. | | precip conc.||2297.7||43.69||1.90 | ||
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| precip conc, 10x dil||205. | | precip conc, 10x dil||205.7||5.06||1.82 | ||
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Revision as of 10:37, 3 June 2015
 DNA Concentration of pMaxGFP
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OverviewThe sample left to dry overnight has evaporated down to a volume of under 30 µL. I'm taking another 500 µL of the plasmid and performing a sodium acetate-ethanol precipitation, following the instructions in the Sigma GenElute Plasmid Maxiprep Kit from yesterday. Final volume of the precipitation concentration will be 100 µL, a theoretical 5-fold increase in concentration. DNA QuantificationI ran each sample in duplicate, and at straight (undiluted) and 10x diluted concentrations.
There's a little bit of saturation of the instrument at concentrations around 2000 ng/µL, but overall the results look very consistent with each other and with expected values. Recovery from the precipitation is 85%.
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