Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/22: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(fix raw html notebook nav) |
||
| Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Transfection optimization of K562</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Transfection optimization of K562</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Latest revision as of 00:58, 27 September 2017
 Transfection optimization of K562
|
 Main project page Previous entry Next entry
|
OverviewMeasured cell density of K562 culture in antibiotic-free medium at 490,000 cells/mL. At 9 mL total culture, that's 4,410,000 cells total. Ideal cell density for transfection with lipofectamine in 24-well plate is 0.5-2E5 cells per well. Assume 1 extra mL of culture as buffer. 4.90E5 cells/mL * x mL = 0.5E5 cells/mL * 25 mL x = 12.5E5 cells / (4.9E5 cells/mL) x = 2.55 mL 25 - 2.55 = 22.45 mL medium
|
|
