Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/26: Difference between revisions

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{|{{table}} width="850"
{|{{table}} width="850"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Today's project is...</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">K562 transfection optimization results</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Overview==
==Overview==
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
| align="center" style="background:#f0f0f0;"|'''DNA (µg)'''
| align="center" style="background:#f0f0f0;"|'''LTX (µL)'''
| align="center" style="background:#f0f0f0;"|'''% transfection (avg)'''
| align="center" style="background:#f0f0f0;"|'''% transfection (st dev)'''
|-
| 1||4||2||2.81%||0.004949747
|-
| 2||1||2||1.50%||0.002404163
|-
| 3||0.4||2||0.50%||0.000636396
|-
| 4||6||3||1.90%||0.003676955
|-
| 5||1.5||3||1.86%||0.000494975
|-
| 6||0.6||3||0.77%||0.000282843
|-
| 7||8||4||4.15%||0.003676955
|-
| 8||2||4||1.52%||0.003889087
|-
| 9||0.8||4||1.12%||0.000565685
|-
| 10||10||5||4.47%||0.001626346
|-
| 11||2.5||5||2.53%||0.002192031
|-
| 12||1||5||2.53%||0.013010765
|-
| 0||0||0||0.08%||0.000494975
|}
[[Image:2015-05-26_K562_test_transfection_image_1.png | 500px]]
[[Image:2015-05-26_K562_test_transfection_image_2.png | 500px]]
There appears to be a direct correlation between amount of DNA used and transfection efficiency. All recorded efficiencies were very low, however (<5%). Amount of LTX does not appear to have a significant effect, although this is difficult to verify as no samples used a constant amount of DNA while varying lipofectamine.


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Latest revision as of 00:59, 27 September 2017

K562 transfection optimization results Main project page
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Overview

Sample ID DNA (µg) LTX (µL) % transfection (avg) % transfection (st dev)
1 4 2 2.81% 0.004949747
2 1 2 1.50% 0.002404163
3 0.4 2 0.50% 0.000636396
4 6 3 1.90% 0.003676955
5 1.5 3 1.86% 0.000494975
6 0.6 3 0.77% 0.000282843
7 8 4 4.15% 0.003676955
8 2 4 1.52% 0.003889087
9 0.8 4 1.12% 0.000565685
10 10 5 4.47% 0.001626346
11 2.5 5 2.53% 0.002192031
12 1 5 2.53% 0.013010765
0 0 0 0.08% 0.000494975

There appears to be a direct correlation between amount of DNA used and transfection efficiency. All recorded efficiencies were very low, however (<5%). Amount of LTX does not appear to have a significant effect, although this is difficult to verify as no samples used a constant amount of DNA while varying lipofectamine.



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