Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/05/26: Difference between revisions
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There appears to be a direct correlation between amount of DNA used and transfection efficiency. All recorded efficiencies were very low, however (<5%). Amount of LTX does not appear to have a significant effect, although this is difficult to verify as no samples used a constant amount of DNA while varying lipofectamine. | |||
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Revision as of 14:55, 26 May 2015
K562 transfection optimization results | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Overview
There appears to be a direct correlation between amount of DNA used and transfection efficiency. All recorded efficiencies were very low, however (<5%). Amount of LTX does not appear to have a significant effect, although this is difficult to verify as no samples used a constant amount of DNA while varying lipofectamine.
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