Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/09/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Transfection Overview</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Transfection Overview</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Latest revision as of 01:06, 27 September 2017
Transfection Overview | Main project page Previous entry Next entry |
OverviewI'm going to be performing a series of transfections with the following parameters: Cell type:
Time points:
Replicates:
Vector used: KAH126-MV2 Other notes:
U-2 OS TransfectionEarlier in the week, I set up a 6-well plate with U-2 OS cells, and today I transfected the plate with KAH126-MV2 using Lipofectamine LTX. Wells 1, 2, 4, and 5 were treated with 4 µg of plasmid, and wells 3 and 6 were negative controls (no plasmid). After transfection, the plate was centrifuged at 128 g for 30 minutes at room temperature before being transferred to the CO2 incubator.
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