Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2016/08/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Today's project is...</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Today's project is...</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Overview== | ==Overview== | ||
Transfected K562 cells on 8/20, following same protocol as before ( | Transfected K562 cells on 8/20, following [http://openwetware.org/wiki/Haynes_Lab:Notebook/RNA-seq_of_PcTF_Transfected_U2OS_%26_SK-N-SH_cell_lines/2015/06/03 same protocol as before] (from 2015-06-03). | ||
==K562 KAH132 Results - Microscopy== | |||
<b>24hr</b> | |||
Well 1:<br> | |||
[[Image:2016-08-21_Well_1_KAH132-MV2_phase_mCherry_10x.jpg | 250px]] | |||
Well 2:<br> | |||
[[Image:2016-08-21_Well_2_KAH132-MV2_phase_mCherry_10x.jpg | 250px]] | |||
Well 4:<br> | |||
[[Image:2016-08-21_Well_4_KAH132-MV2_phase_mCherry_10x.jpg | 250px]] | |||
Well 5:<br> | |||
[[Image:2016-08-21_Well_5_KAH132-MV2_phase_mCherry_10x.jpg | 250px]] | |||
----- | |||
<b>48 hr</b> | |||
Well 2:<br> | |||
[[Image:2016-08-22_Well_2_KAH132-MV2_phase_mCherry_10x.jpg | 250px]] | |||
Well 5:<br> | |||
[[Image:2016-08-22_Well_5_KAH132-MV2_phase_mCherry_10x.jpg | 250px]] | |||
==K562 KAH132 Results - Flow Cytometry== | |||
24 hour:<br> | |||
[[Image:Screen_Shot_2016-09-20_at_11.10.45_AM.png]] | |||
48 hour:<br> | |||
[[Image:Screen_Shot_2016-09-20_at_11.10.50_AM.png]] | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample ID''' | |||
| align="center" style="background:#f0f0f0;"|'''% RFP''' | |||
|- | |||
| 24hr A||84.8 | |||
|- | |||
| 24hr B||84.9 | |||
|- | |||
| 48hr A||28 | |||
|- | |||
| 48hr B||26 | |||
|} | |||
==K562 RNeasy prep== | |||
Harvested cells today, RNeasy prep. Concentrations/calculations for cDNA synthesis: | Harvested cells today, RNeasy prep. Concentrations/calculations for cDNA synthesis: |
Latest revision as of 01:52, 27 September 2017
Today's project is... | Main project page Previous entry | |||||||||||||||||||||||||||||||||||
OverviewTransfected K562 cells on 8/20, following same protocol as before (from 2015-06-03). K562 KAH132 Results - Microscopy24hr
48 hr
K562 KAH132 Results - Flow Cytometry
K562 RNeasy prepHarvested cells today, RNeasy prep. Concentrations/calculations for cDNA synthesis:
Following steps from cDNA synthesis protocol.
|