Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2016/08/22

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Overview

Transfected K562 cells on 8/20, following same protocol as before (enter date).

Harvested cells today, RNeasy prep. Concentrations/calculations for cDNA synthesis:

Sample ID ng/µL µL for 2 µg RNA (8 µL max) µL H2O qPCR Sample ID
K562 KAH132 24hr 1 917 2.2 5.8 K562_E008
K562 KAH132 24hr 2 837 2.4 5.6 K562_E009
K562 KAH132 48hr 1 879 2.3 5.7 K562_E010
K562 KAH132 48hr 2 638 3.1 4.9 K562_E011

Following steps from cDNA synthesis protocol.



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